Question

How do you find K_m and V_max?

Answer 1

Km and Vmax:

1. V_max is the maximum reaction velocity at which all enzymes become saturated with substrate.
2. K_m is the substrate concentration at which half of the maximum velocity is achieved.
3. We can determine V_max and K_m for a reaction by plotting a graph of the rate of reaction (v) against the concentration of substrate [S].