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Question

How many selectable markers are present in pBR322?


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Solution

Selectable marker:

  1. The selectable marker could be a gene present within the vector that, when introduced into the host or cells in culture, results in a trait suitable for artificial selection.
  2. Selectable markers for antibiotic resistance are commonly used.
  3. These aid in the artificial selection process.

Selectable markers in pBR322:

  1. The cloning vector pBR322 contains selectable markers for ampicillin and tetracycline resistance genes.
  2. They play a supportive role in the differentiation of transformed cells from non-transformed cells.
  3. They also aid in distinguishing between recombinant and non-recombinant cells.


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