Question

How to identify a PCR product?

Answer 1

PCR (polymerase chain reaction) product:

1. The identification of evolutionary links between species can be done using a modification of one of the PCR techniques.
2. The size of the PCR product may be determined, as well as its composition using agarose gel electrophoresis.
3. This technique involves inserting DNA into an agarose gel and applying an electric current to the gel afterward.
4. As a result, the smaller DNA strands tend to travel through the gel more quickly than the bigger strands in the direction of the positive current.
5. By comparing the size of the PCR result to a DNA ladder made up of known-sized DNA fragments in the gel, the size of the PCR product can be determined.