Question

In the Lambda Repressor binding, how is integration possible?

Answer 1

Lambda Repressor Binding:

1. In Lambda repressor binding the integration happens when the cII protein starts the transcription of the ‘int’ gene and c1 gene simultaneously.
2. The function of the int gene is the production of an integrase enzyme and thus the protein amount increases before the lambda transcription is switched off.
3. The DNA of lambda comprises a phage attachment site with the help of which the lambda could pair with the bacterial attachment site found between the gal-operon and bio-operon on the chromosome of the E-coli.
4. When both of these sites are matched, the integrase enzyme and the specialized protein start the physical exchange between bacterial and viral DNA strands.