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Question

Mention ways in which viruses can be purified.?


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Solution

Viruses:

  1. With the help of gradient centrifugation, viruses might be isolated based on their density and size.
  2. The concentration of a sucrose solution is allowed to grow linearly and smoothly between the bottom and top of a centrifuge tube.
  3. The virus preparation is usually put on top of the gradient and centrifuged after being purified by differential centrifugation.
  4. The particles settle under the centrifugal force until they find rest at the point where the gradient's density equals their own.
  5. If separation is desired, only a modest difference in density between viruses and other particles is required.

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