Question

The sequence that controls the copy number of the linked DNA in the vector, is termed?

• A. Palindromic Sequence
• B. Recognition Site
• C. Selectable Marker
• D. Ori Site

Answer 1

The correct option is D

Ori Site

The correct option is D.

Explanation for the correct option:

1. Ori, which stands for "Origin of Replication," is a sequence from which replication begins.
2. The ori is where DNA replication starts, allowing plasmids to replicate themselves in order to live inside cells.
3. Plasmids utilize the host's machinery to create extra copies even though their replicons often differ from those used to replicate the chromosomal DNA of the host.

Explanation for the incorrect options:
Option A:

When read from the same direction on both strands—either 5' to 3' or 3' to 5'—on both the DNA duplex and the RNA, the sequence in one strand is the same as the corresponding sequence of the other strand, which is known as a palindromic sequence.

Option B:

1. These areas are referred to as recognition sequences or recognition sites in restriction enzymes and are dispersed randomly throughout the DNA.
2. Restriction enzymes produced by various bacterial species may detect various nucleotide sequences.

Option C:

1. The altered cells can be chosen using a selectable marker.
2. These markers typically confer resistance to phototoxic substances, such as antibiotics and herbicides.
3. It is an essential component of the transformation vector and a stable dominant gene.

Final answer:
The sequence that controls the copy number of the linked DNA in the vector, is termed as Ori.