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Question

To obtain virus-free healthy plants from a diseased one by tissue culture technique, which part/parts of the diseased plant will be taken?


A

Epidermis only

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B

Apical meristem only

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C

Both apical and axillary meristems

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D

Palisade parenchyma

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Solution

The correct option is C

Both apical and axillary meristems


The correct option is C.

Explanation of the correct option:

  1. Even if the plant gets some viral infection still the meristematic region remains free.
  2. Hence, meristems are taken out from the plant and are grown in vitro to obtain a plant that is free from viruses.
  3. Therefore, both axillary and apical meristems are used for this purpose.

Explanation of the incorrect options:

Option A:

The epidermis of the plant gets infected by the virus easily thus it cannot be used for the tissue culture process.

Option B:

Along with the apical meristem, the axillary meristem is also used for tissue culture.

Option D:

Parenchyma isn't part of the meristem so it gets infected with the virus and thus, it is unhealthy for tissue culture.

Final answer: To obtain virus-free healthy plants from a diseased one by tissue culture technique, both apical and axillary meristems of the diseased plant will be taken.


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