Question

What are selectable markers and what is their use in genetic engineering?

Answer 1

Selectable markers and their use in genetic engineering

1. The selectable marker is a DNA sequence that aids in detecting and eliminating non-transformants while allowing transformants to grow selectively.
2. For this purpose, the vector needs a selectable marker.
3. The ability to select marker genes is an essential aspect of most transition regimens.
4. They are either released on the same plasmid as the gene of interest or on a separate plasmid.
5. A selectable marker is required for the plasmid's maintenance in the cell.
6. The plasmid becomes helpful to the cell due to the presence of a selection marker.
7. Under selective conditions, only cells harbouring plasmids containing the appropriate selectable marker will survive.
8. In the cloning of vectors, genes that provide resistance to various antibiotics are commonly utilized as selection markers.