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Question

What are the three initiation factors used by bacteria to enter the initiation complex?


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Solution

Initiation factor:

  1. The proteins that enable RNA polymerase II to bind to DNA template in order to start transcription.
  2. Bacteria use three initiation factors for tRNA and mRNA to enter the initiation complex,

Initiation factors used by bacteria to enter the initiation complex are as follows:

  1. IF-1: The initiation factor 1 involves 30S subunits as a portion of the complete initiation complex. It associates with the A site preventing entry of aminoacyl-tRNA. The binding of the 30S subunit with the 50S subunit can be hindered by its location.
  2. IF-2: The initiation factor 2 involves special initiator tRNA regulating their entry to the ribosome.
  3. IF-3: The initiation factor 3 is required for 30S subunits to specifically associate with the initiation sites in the mRNA.

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