Question

What determines where restriction enzymes cut?

Answer 1

Restriction enzyme:

1. Restriction enzyme, also known as restriction endonuclease, is a protein produced by bacteria that cleaves DNA at specific sites along the molecule.
2. In the bacterial cell, restriction enzymes cleave foreign DNA and will be eliminating infecting organisms.

The factors that determine the site where restriction enzymes are cut are discussed below:

1. The restriction site is a specific DNA sequence that has a special palindromic sequence complementary to the sequence of the enzyme.
2. When restriction enzymes come into contact with this DNA sequence called the recognition site, it wraps around the DNA and creates a cut in both DNA strands of the DNA molecule.
3. Each restriction enzyme recognizes a different and specific recognition site or DNA sequence.