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Question
Explain Hot start PCR.
Explain Hot start PCR.
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Solution
Hot start PCR:
- Hot start PCR is a technique that begins the polymerase reaction at or above the annealing temperature of the primer.
- This eliminates the possibility of non-specific annealling of primer extensions at ambient temperature where the Taq Pol retains partial activity.
- High yield and specificity of the intended gene product can be achieved with hot start PCR.
- The polymerase should be added when the mixture reaches a temperature higher than the annealing temperature to achieve the hot start PCR condition.
Hot start PCR:
- Hot start PCR is a technique that begins the polymerase reaction at or above the annealing temperature of the primer.
- This eliminates the possibility of non-specific annealling of primer extensions at ambient temperature where the Taq Pol retains partial activity.
- High yield and specificity of the intended gene product can be achieved with hot start PCR.
- The polymerase should be added when the mixture reaches a temperature higher than the annealing temperature to achieve the hot start PCR condition.
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