Question

What is needed for polymerase chain reaction?

Answer 1

Polymerase Chain Reaction (PCR):

It is a process used to amplify or make lots of copies of a particular segment of DNA in the laboratory.

For PCR, the following things are needed:

1. Required DNA segment.
2. DNA polymerase enzyme (thermodynamically stable).
3. DNA primers or oligonucleotide primers.
4. Optimum system for the process should include a buffer system.
5. Free nucleotide triphosphates.