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Question

What is PCR cloning used for?


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Solution

PCR:

  1. PCR is the polymerase chain reaction.
  2. This is a method for cloning a gene or for the amplification of a gene to make multiple copies.

Steps in PCR:

  1. Denaturation: At temperatures between 94°-95°C, here denaturation of DNA occurs. The parent DNA breaks and forms two strands due to application of high temperature.
  2. Annealing: At temperature between 50°-56° C, the DNA primer bind to the DNA strands.
  3. Elongation: At a temperature of 72° C, the Taq polymerase bind to primer and forms double-stranded DNA from a single strand.

Uses of PCR cloning:

  1. Human diagnostic: Detection of infection (viral, bacterial, fungal).
  2. Environmental monitoring: Water quality monitoring and food safety testing.
  3. Scientific Research: Preparing DNA to sequence, Monitoring gene expression, Manipulating DNA in genetic engineering and synthetic biology.


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