Question

Which of the given enzymes is not involved during DNA replication on the template with polarity 3' → 5'?

• A. DNA polymerase
• B. Helicase
• C. DNA Ligase
• D. None of the above

Answer 1

The correct option is C

DNA Ligase

The correct option is C.

Explanations of the correct option:

1. The enzyme known as DNA ligase facilitates the synthesis of a phosphodiester bond, which facilitates the joining of DNA strands.
2. It helps to repair single-strand breaks in duplex DNA in living creatures, even though some forms may only fix double-strand breaks.
3. They are unable to start the creation of new DNA.

Explanations of the incorrect options:

Option A:

1. DNA replication is the primary method by which DNA polymerase creates new DNA.
2. Deoxyribonucleotides, the building blocks of DNA, are synthesised into DNA molecules by an enzyme known as DNA polymerase.
3. These enzymes work in pairs to divide a single original DNA molecule into two identical DNA strands, which is necessary for DNA replication.
4. DNA polymerase works in pairs, replicating two strands of DNA in tandem.

Option B:

1. DNA helicase's primary job is to split two DNA strands apart for replication.
2. The enzyme known as helicase is responsible for releasing the hydrogen bonds holding the DNA strands together.
3. As a result, it aids in the replication fork's creation.

Final answer: DNA Ligase is not involved during DNA replication on the template with polarity 3' → 5'.