Definition:
- pUC18 is a plasmid cloning vector.
- It was synthesized at the University of California.
Invented by: Yanisch-Perron et al created pUC18.
Applications:
- E.coli is used in replication as puc18 is a small, high copy cloning vector.
- It has been constructed by using ampicillin resistance gene and pMB1 origin of replication from pBR22.