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Question

Why is restriction endonuclease so called?


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Solution

Restriction endonuclease:

  1. Endonuclease means by cleaving the internal covalent connections joining nucleotides, this enzyme breaks down a nucleotide chain into two or shorter strands.
  2. Restriction endonucleases are named from the fact that they stop bacteriophages from multiplying by recognizing and cutting DNA at specified locations.
  3. Every endonuclease looks for the palindromic recognition sequence throughout the complete DNA sequence.
  4. Restriction enzymes break DNA at or near certain recognition sequences known as restriction sites, earning them the nickname “molecular scissors.”

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