Question

[3]

a) Name the process represented by the above diagram.
b) What is the principle behind this experiment?
c) How are the bands observed?

Answer 1

a) The process which is represented in the diagram is agarose gel electrophoresis. [1]

b) The principle used in this process is separation of DNA fragments based on their size through an electric field. Smaller fragments move faster. [1]

c) The bands are observed after staining it with a compound known as ethidium bromide followed by exposure to UV radiation. [1]