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Ligation of DNA

3. What will ...

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3. What will happen, if the restriction enzymes have more than one recognition site in a vector?

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Solution

Dear student,
If restriction enzymes have more than one restriction sites in a vector then it will cleave at more than one site in a vector so desired site of integration of gene will not occur which will make cloning and genetic engineering difficult.

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Q. Assertion :The cloning vector is required to have very few, preferably single recognition commonly used used restriction enzymes. Reason: Presence of more than one recognition site within a cloning vector will generate which will complicate the process of gene cloning.

Q. Read the given statements and select the correct option.
Statement 1 : The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.Statement 2 : Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.

Q. Which among the following are features required in a vector to facilitate cloning?
I. It should have ori - a sequence from where replication starts.
II. It should have a selectable marker.
III. It should have several recognition sites for a particular restriction enzyme.
IV. It should have a single recognition site for commonly used restriction enzymes.

Q. How many fragments will be generated if you digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA?

Recognition sites for commonly used restriction enzymes meaning

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