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Question

77. Consider the following statement about CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)
1. It is a genome editing technique.
2. This can be used to correct mutations at precise locations in the human genome.
3. Single-guide RNA (sgRNA) is one of the components of the CRISPR.
Correct answer code is?

A
Only 1 and 2
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B
Only 2 and 3
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C
Only 1 and 3
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D
All of the above
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Solution

The correct option is D All of the above

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats.
CRISPR - Cas9 is the most prominent genome editing technique.
It allows researchers to permanently modify genes in living cells and organisms.
This can be used to correct mutations at precise locations in the human genome to treat genetic causes of diseases.
Correcting the mutation in an embryo ensures that the child is born healthy and the defective gene is not passed on to future generations.
The gene editing tool has two components: a single-guide RNA (sgRNA) that contains a sequence that can bind to DNA. The Cas9 enzyme which acts as a molecular scissor that can cleave DNA.
In order to selectively edit a desired sequence in DNA, the sgRNA is designed to find and bind to the target.


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