Bacterial Synthesis of Insulin (Human)

Q. The main challenge for the production of insulin using rDNA technique was

1. Joining chain A with C peptide chain
2. Getting insulin assembled into its mature form
3. Separating chain A and chain B
4. Removing B peptide chain

Q. Which hormone is injected into diabetes patient and why

Q. In the bacterial synthesis of humulin, the plasmid containing the human insulin gene is introduced into _____

1. E.coli
2. Bt
3. Pneumococcus
4. None of these

Q. Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli

1. ii-i-iv-iii-v-vi
2. i-iii-v-vi-ii-iv
3. i-iv-v-ii-vi-iii
4. iii-v-ii-i-vi-iv