ELISA

Q. AIDS can be detected by

1. Northern blotting
2. Southern blotting
3. Western blotting
4. ELISA

Q. ELISA can give a false test in the beginning of an infection due to

1. Denatured pathogen
2. Antibody may not bind tightly to the titer plate
3. Low concentration of pathogen
4. Low antibody

Q. AIDS cannot be transmitted by ______.

1. Hugs
2. Breast feeding
3. Blood tansfusion
4. Sexual contact

Q. ELISA is used to detect viruses where the key reagent is:

1. Alkaline phosphatase
2. Catalase
3. DNA probe
4. RNase

Q. Match the images and the steps of ELISA

a. A substrate is present which the enzyme cleaves and a colored reaction is produced
b. Enzyme linked antibody binds to its specific complementary antigen.
c. Blood serum from the patient is added and incubated for 30 minutes (wash)
​d. The well is first coated with an antigen and then washed

1. 
2. 
3. 
4. 

Q. ELISA is based on

1. sequence of amino acids in a protein
2. Antigen antibody interaction
3. probe binding
4. hybridization

Q. ELISA is used to detect viruses, where

1. Catalase is the key reagent.
2. Southern blotting is done.
3. DNA-probes are required.
4. Alkaline phosphatase is the key reagent.

Q. name three molecular diagnostic technique that help detect pathogens from suspected patients . give one advantage of these technique over conventional method