PCR Cycle
Trending Questions
Q. The extension step of PCR where DNA polymerase gets into action happens at 72∘C. This is a contrast to what we have learnt where enzymes are said to be functioning at optimum temperature range of 35∘ to 40∘C. Why is it so?
- DNA polymerase is active at low pH and high temperature
- While
is the optimum temperature range,
is a special sweet spot for most enzymes to work
- The temperature has no role to play in this process.
- DNA polymerase used in PCR is obtained from a bacteria that thrives in hot springs
Q. DNA polymerase can be obtained from
- pBR 322
- Thermus aquaticus
- Agrobacterium
- Retro virus
Q. The instrument used for PCR is called __ .
Q.
PCR can be used for :
DNA fingerprinting
Detection of pathogens
Diagnosis of a specific mutation.
All of the above