Ti Plasmid

Q. What is bt gene?

Q.

Which one of the following proved effective for biological control of nematode disease in plants?

1. Gliocladium virens

2. Pseudomonas cepacia

3. Pisolithus tinctorius

4. Paecilomyces lilacinus

Q.

The vector of T-DNA is

1. Salmonella typhimurium

2. Thermus aquaticus

3. Agrobacterium tumefaciens

4. Escherichia coli

Q. Agrobacterium naturally inserts DNA into plants via the

1. Antibiotic resistant gene
2. Ti plasmid
3. Cosmid
4. Marker gene

Q. Wild Ti plasmid is able to induce tumors in plants due to the presence of:

1. Cancer genes
2. Kanamycin resistance gene
3. Phytohormone coding genes
4. Cry genes

Q.

Which of the following is gentically engineered into crops to make them resistant to Insects?

1. Aeromonas hydrophila

2. Lactobacillus

3. Bacillus anthrasis

4. Bacillus thuringiensis

Q. The Ti-plasmid is used for making transgenic plants. This plasmid is found in

1. Azotobacter
2. Rhizobium of the roots of leguminous plants
3. Agrobacterium
4. Yeast as a 2µm plasmid

Q. Wild Ti plasmid is able to induce tumors in plants due to the presence of:

1. Phytohormone coding genes
2. Kanamycin resistance gene
3. Cry genes
4. Cancer genes

Q. Bacillus thuringiensis (Bt) strains have been used for designing novel

1. Bioinsecticidal plants
2. Biomineralization processes
3. Biofertilizers
4. Biometallurgical techniques

Q. The bacteria associated with plant genetic engineering are:

1. Salmonella and Pseudomonas
2. Salmonella typhimurium and Agrobacterium tumefaciens
3. Bacillus thuringiensis and Pseudomonas fluorescens
4. Rhizobium leguminosarum and Azotobacter agilis

Q. Transgenes are transferred to plants using Ti plasmid present in:

1. Salmonella typhimurium
2. Agrobacterium tumefaciens
3. Bacillus thuringiensis
4. Pseudomonas fluorescens

Q. Which of the following modifications are made to make a “tamed” Ti plasmid which can transfer cry genes to a plant’s genome?

1. Removal of kanamycin resistance gene and adding phytohormone genes
2. Removal of phytohormone gene and adding kanamycin resistance gene
3. Removal of both kanamycin resistance gene and phytohormone genes
4. Adding both phytohormone and kanamycin resistance gene

Q. Which one of the following is commonly used in the transfer of foreign DNA into crop plants?

1. Penicillium expansum
2. Trichoderma harzianum
3. Meloidogyne incognita
4. Agrobacterium tumefaciens

Q.

The vector of T-DNA is

1. Thermus aquaticus

2. Salmonella typhimurium

3. Agrobacterium tumefaciens

4. Escherichia coli

Q. In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?

1. To make the leaf round shape
2. To induce induce dedifferentiation in leaf cells
3. To increase surface area for transfers
4. To use both surfaces of the leaf

Q. Which of the following represents the correct sequence of steps in leaf disc transfer method?

1. Dedifferentiation cutting leaf discs redifferentiation agrobacterium infection
2. Cutting leaf discs dedifferentiation agrobacterium infection redifferentiation
3. Agrobacterium infection redifferentiation cutting leaf discs dedifferentiation
4. Cutting plant discs agrobacterium infection dedifferentiation redifferentiation

Q. The disease root-knot of brinjal is caused by

1. Fusarium udum
2. Phytophthora infestans
3. Meloidogyne incognita
4. Pseudomonas rubrilineans
5. Xanthomonas citri