What is PCR

Q.

The basis of DNA fingerprinting is

1. The double helix

2. Error in base sequence

3. Polymorphism in sequence

4. DNA replication

5. DNA coiling

Q.

Write a full form of RFLP

Q. What is the main reason for PCR to be employed in rDNA technology?

1. DNA isolated from a cell sample will always be very less
2. PCR increase the chance of DNA isolation
3. Sometimes the DNA extraction from certain cells is very tough
4. PCR helps in identifying the gene of interest very easily

Q. Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only a little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is by

1. hybridising the DNA with a DNA probe
2. subjecting the DNA to polymerase chain reaction
3. subjecting the DNA to gel electrophoresis
4. treating the DNA with restriction endonucleases

Q. In vitro clonal propagation in plants in characterized by:

1. PCR and RAPD
2. Northern blotting
3. Electrophoresis and HPLC
4. Microscopy