PCR Cycle
Trending Questions
Q.
What is PCR cloning used for?
Q. The thermostable enzymes, ‘Taq’ and ‘Pfu’, isolated from thermophilic bacteria are
- RNA polymerases
- DNA polymerases
- restriction endonucleases
- DNA ligases
Q. Why does PCR amplify DNA strands so rapidly?
- PCR includes oxidation of DNA
- PCR causes DNA bases to multiply
- PCR is an addition reaction
- PCR is an exponential reaction
Q. Which of the following scientists won the Nobel prize for the development of Polymerase chain rection (PCR) in the year 1993
- Cesar Milstein
- Philip Sharp
- Susumu Tonegava
- Kary Mullis
Q. What is the most important constituent of Polymerase Chain Reaction?
- DNA polymerase
- DNA primers
- DNA Sequence
- All the above components
Q. The Taq polymerase enzyme is obtained from :
- Thermus aquaticus
- Thilobacillus ferroxidans
- Bacillus subtilis
- Pseudomonas putida
Q.
The cutting of DNA at specific locations became possible with the discovery of:
Probes
Restriction enzymes
Selectable markers
Ligases
Q. Palaeontologists unearthed a human skull during excavation. A small fragment of scalp tissue was still attached to it. Only little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is:
- By hybridizing the DNA with the DNA probe
- By subjecting the DNA into gel electrophoresis
- By subjecting the DNA into polymerase chain reaction
- By treating the DNA with restriction endonucleases
