What Is PCR

Q.

Why are cheek cells used for DNA extraction?

Q.

What are the steps of DNA fingerprinting?

Q. PCR and restriction fragment length polymorphism are the methods for

1. study of enzymes
2. genetic transformation
3. DNA sequencing
4. genetic fingerprinting

Q.

What does DNA fingerprinting involve?

Q. The polymerase Chain Reaction (PCR) is technique that is used for

1. in vivo replication of specific DNA sequence using thermostable DNA polymerase
2. in vivo synthesis of mRNA
3. in vitro replication of specific DNA sequence using thermostable DNA polymerase
4. separation of DNA fragments according to their size
5. in vitro synthesis of mRNA

Q.

How to identify a PCR product?

Q. Why does PCR amplify DNA strands so rapidly?

1. PCR causes DNA bases to multiply
2. PCR includes oxidation of DNA
3. PCR is an addition reaction
4. PCR is an exponential reaction

Q. PCR and restriction fragment length polymorphism are the methods for

1. study of enzymes
2. genetic transformation
3. DNA sequencing
4. genetic fingerprinting

Q. What is the main reason for PCR to be employed in rDNA technology?

1. DNA isolated from a cell sample will always be very less
2. Sometimes the DNA extraction from certain cells is very tough
3. PCR increase the chance of DNA isolation
4. PCR helps in identifying the gene of interest very easily

Q. Which of the follwoing is true regarding the DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells.
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature

Q. why is PCR also known as thermal cycling?

Q. In vitro clonal propagation in plants in characterized by:

1. Northern blotting
2. Electrophoresis and HPLC
3. Microscopy
4. PCR and RAPD

Q. Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only a little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is by

1. subjecting the DNA to polymerase chain reaction
2. subjecting the DNA to gel electrophoresis
3. treating the DNA with restriction endonucleases
4. hybridising the DNA with a DNA probe

Q. The polymerase Chain Reaction (PCR) is technique that is used for

1. in vitro synthesis of mRNA
2. in vivo replication of specific DNA sequence using thermostable DNA polymerase
3. in vitro replication of specific DNA sequence using thermostable DNA polymerase
4. separation of DNA fragments according to their size
5. in vivo synthesis of mRNA

Q. The purpose of polymerase chain reaction is __________

1. DNA replication
2. DNA visualization
3. DNA modification
4. DNA amplification

Q. The Polymerase chain reaction (PCR) is a powerful technique to

1. Amplify genes
2. Inhibit DNA synthesis
3. Mutate Genes
4. Induce protein synthesis

Q.

PCR is required for

1. DNA synthesis

2. DNA amplification

3. Protein synthesis

4. Amino acid synthesis