DNA Replication

Q. Which of the following enzymes are required for DNA replication?

1. DNA polymerase
2. RNA polymerase
3. DNA ligase
4. Helicase

Q. What is the importance of DNA replication?

Q. During replication of a bacterial chromosomes DNA synthesis starts from a replication origin site and:

1. is facilitated by telomerase
2. moves in one direction
3. moves in bi-directional way
4. RNA primers are involved

Q. What is the direction of synthesis of DNA by DNA Polymerase?

1. 3' to 5'
2. 5' to 3'
3. No set direction for DNA Polymerase
4. Direction is decided by the Ori sequence

Q.

Which of the following is not an enzyme involved in DNA replication?

1. Helicase
2. DNA Polymerase
3. Ligase
4. RNA primer

Q.

A DNA strand is directly involved in the synthesis of all of the following except:

1. DNA molecule

2. Protein synthesis

3. mRNA molecule

4. tRNA molecule

Q. In E. coli, during lactose metabolism, the repressor binds to ________.

1. Regulator gene
2. Operator gene
3. Structural gene
4. Promoter gene

Q.

Which of the following phases involve the replication of DNA in preparation for mitosis?

1. R phase

2. S phase

3. G1 phase

4. G2 phase

Q.

During replication of bacterial DNA, synthesis starts from a replication origin site and:

1. Is facilitated by telomerase

2. Moves in one direction

3. Moves in a bi-directional way

4. RNA primers are involved

Q.

Match the following enzymes of DNA replication with their respective functions:
$\begin{array}{cc}p.DNApolymerase& i.HelpsunziptheDNAdoublehelix\\ q.DNAligase& ii.SealsnicksbetweenOkazakifragments\\ r.DNAhelicase& iii.LaysRNAprimerstowhichpolymerasecanaddnucleotides\\ s.Primase& iv.Addsnucleotidestothefree{3}^{\prime }endofRNAprimers\end{array}$

1. p-i, q-ii, r-iii, s-iv

2. p-iii, q-i, r-ii, s-iv

3. p-iv, q-ii, r-i, s-iii

4. p-ii, q-iii, r-iv, s-i

Q. When DNA replication begins, which of the following happens?

1. The bond between the nitrogen base and sugar of each nucleotide breaks.
2. The hydrogen bonds between the nucleotides forming a base pair break.
3. The phosphodiester bonds between adjacent nucleotides break.
4. DNA ligase unzips the double helix.

Q. DNA can be formed by

1. RNA dependent DNA polymerase
2. All of the above
3. Transaminase
4. Lyases

Q.

The number of DNA strands in a chromosome at the G2 stage is

1. One

2. Two

3. Four

4. Eight

Q. What is the function of the enzyme DNA Helicase in DNA Replication?

Q. During DNA replication, the strands are separated by ________.

1. DNA polymerase
2. Topoisomerase
3. Helicase
4. Gyrase

Q. Name the following:
Formation of new DNA.

Q.

Which among the following is true regarding the replication of DNA?

1. Happens before the mitotic division

2. Happens after karyokinesis

3. Happens during mitotic stage

4. Happens in the daughter cells

Q.

Duplication of DNA occurs in ________ stage of a cell.

Q. The Okazaki fragments in DNA chain growth:

1. polymerize in the 5' - 3' direction and explain 3' - 5' DNA replication
2. result in transcription
3. polymerize in the 3'-to-5' direction and forms replication fork
4. prove semi-conservative nature of DNA replication

Q. The difference between RNA and DNA is because of ________.

1. Sugar and base
2. Sugar and phosphate
3. Sugar only
4. Phosphate and base

Q.

What is DNA replication?

1. The process during which the hydrogen bonds between the nucleotides of two strands break

2. The process during which DNA strand winds around a core of histone molecules

3. The process by which a double-stranded DNA molecule is copied into two, identical DNA molecules.

4. The process during which the bonds between the nitrogen base and deoxyribose sugar break

Q.

The Okazaki fragments in DNA chain growth:

1. polymerize in the 5' - 3' direction and explain 3' - 5' DNA replication

2. result in transcription

3. polymerize in the 3'-to-5' direction and forms replication fork

4. prove semi-conservative nature of DNA replication

Q. Whether or not there is DNA synthesis in head interkinesis

Q.

Which of the following enzymes are required for DNA replication?

1. DNA polymerase

2. RNA polymerase

3. DNA ligase

4. Helicase

Q.

DNA can be formed by

1. Transaminase

2. RNA dependent DNA polymerase

3. Lyases

4. All of the above

Q. dNTPs differ in the structure from the normal NTPs and therefore stop the polymerase from moving forward in the replication fork and continue the replication process.

1. True
2. False

Q.

Match the following enzymes of DNA replication with their respective functions:
$\begin{array}{cc}p.DNApolymerase& i.HelpsunziptheDNAdoublehelix\\ q.DNAligase& ii.SealsnicksbetweenOkazakifragments\\ r.DNAhelicase& iii.LaysRNAprimerstowhichpolymerasecanaddnucleotides\\ s.Primase& iv.Addsnucleotidestothefree{3}^{\prime }endofRNAprimers\end{array}$

1. p-i, q-ii, r-iii, s-iv

2. p-iii, q-i, r-ii, s-iv

3. p-iv, q-ii, r-i, s-iii

4. p-ii, q-iii, r-iv, s-i

Q.

Okazaki fragments are formed during:

1. Replication

2. Transduction

3. Transcription

4. Translation

Q.

What direction does DNA replication occur along?

1. 3’$\to$5’

2. 5’$\to$3’

3. Any direction

4. No specific direction

Q.

Which of the following is true about spindle fibres?

1. Chromosomal fibres

2. Interzonal fibres

3. Continuous fibres

4. Interchromosomal fibres