Gene Therapy

Q.

What are the applications of biotechnology in the field of agriculture and medicine?

Q. A technique of micropropagation is:

1. Embryo rescue
2. Somatic hybridization
3. Somatic embryogenesis
4. Protoplast fusion

Q.

What antibiotic-resistant genes are in pBR322?

Q.

Which one of the following is a case of wrong matching?

1. Callus - Unorganised mass of cells produced in tissue culture

2. Somatic hybridization - Fusion of two diverse cells

3. Vector DNA - site for tRNA synthesis

4. Micropropagation - In vitro production of plants in large numbers

Q.

How are bacteria able to produce Human insulin in Recombinant DNA Technology ?

Q.

Which of the following is/are used in DNA fingerprinting?

1. Hair strands
2. Fingerprints
3. Blood samples
4. Footprints

Q.

How do plasmids contribute to antibiotic resistance?

Q.

How are polyclonal antibodies derived?

Q. The technique used in biotechnology is

1. Recombinant DNA technology
2. hybridization
3. Both a & b
4. None of these

Q.

Collection of methods used for correction of a gene defect responsible for a disease is called__________

1. DNA fingerprinting

2. genetic engineering

3. gene therapy

4. gene mapping

Q. Explain how insulin can be produced using recombinant DNA technology.

Q.

DNA fingerprinting is useful in

1. Identify criminal

2. To find the identity of the father of any child

3. All of the above

4. Forensic science

Q.

DNA finger is useful in

1. Forensic science

2. All of the above

3. Identify criminal

4. to find the identity of the father of any child

Q.

Identify the incorrect statement regarding gene therapy.

1. Gene therapy is an artificial method that introduces DNA into the cells of human body.

2. Gene therapy is employed to treat diseases such as cancer.

3. Gene therapy is employed to treat diseases such as cold, fever etc.

4. Gene therapy helps to cure the disease causing genes.

Q.

How is fingerprinting used?

Q. a) Define the term prophylaxis.
b) Name the four categories of materials used for preparing vaccines. [5 MARKS]

Q. What is DNA deficiency? Which disease is associated with it?

Q.

Genetically engineered bacteria are being used in the commercial production of_______.

1. Melatonin

2. None of these

3. Human insulin

4. Thyroxine

Q.

The research center for DNA fingerprinting and diagnostic is at

1. Chennai

2. Delhi

3. Hyderabad

4. Mumbai

Q.

Which of the following technique is used to cure a disease by correcting defective genes?

1. Gene therapy

2. Radiation therapy

3. Gene mapping

4. Natural therapy

Q.

Diabetes can’t be treated by the biotechnologically produced insulin.

1. True

2. False

Q. Markers are usually antibiotic __ genes.

Q. Why is the introduction of genetically engineering lymphocytes into a ADA deficiency patient not a permanent cure?

1. Lymphocytes not immortal
2. Lymphocytes not mortal
3. Lymphocytes are integral part of humoral immunity.
   
4. None of the above
   

Q. In genetic engineering, the antibiotics are used

1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers

Q.

The Vaccine that is more thermostable and remains active for the longer duration

1. BCG

2. Vaccines of Polio

3. Hepatitis

4. All of the Above

Q.

What is the first step in gene therapy to treat ADA deficiency?

1. Introduction of a cDNA coding for functional ADA gene into the lymphocytes.

2. Production of genetically engineered lymphocytes.

3. Blood transfusions using genetically engineered lymphocytes.

4. Artificial culturing of the lymphocytes from the patient.

Q. is commercially used to increase the length of plants.

1. Gibberellin
2. Auxin
3. cytokinin
4. Ethylene

Q.

If rDNA is introduced in a gene coding for ampicillin resistance, then

1. The host will lose resistance to ampicillin.

2. The host will become resistant to ampicillin.

3. The host will start producing tetracycline.

4. The host will start producing ampicillin.

Q.

The term “antibiotic” was coined by

1. Alexander fleming

2. Edward Jenner

3. Louis Pasteur

4. Selman waksman

Q. What do you understand by the following terms? [5 MARKS]
(a) Health
(b) Antigens
(c) Innate immunity
(d) Gamma globulin
(e) Antitoxin