Genetic Engineering

Q.

Which of the following is a restriction endonuclease?

1. DNase $\mathrm{I}$

2. RNase I

3. Hind $\mathrm{II}$

4. Protease

Q.

What is the main goal of the Human Genome Project?

Q.

Question 30
What is a clone? Why do offsprings formed by asexual reproduction exhibit remarkable similarity?

Q. During preparation of bread, fermentation of sugar is carried out by yeast which generates .

1. carbon dioxide
2. nitrogen
3. oxygen

Q.

How is biotechnology used today?

Q. Adenine, Guanine, Thymine and Cytosine are the components of .

1. proteins
2. nucleic acids
3. sugars

Q.

What is the enzyme used to anneal the sticky ends of a plasmid to an inserted gene?

Q.

Restriction enzymes present in several micro-organisms cut foreign DNA at specific sites and destroy them. These enzymes do not destroy cellular DNA because______

1. cellular DNA does not have the specific sites recognized by these enzymes

2. the susceptible specific sites are masked by protiens

3. the restriction enzyme susceptible sites are modified by cellular enzymes

4. the restriction enzymes and DNA occupy different compartments

Q.

Question 2 (a)
Yeast is used in the production of :
a) Sugar
b) Alcohol
c) HCl
d) Oxygen

Q. Which of the following are tools used for genetic engineering?

1. Enzymes
2. Markers
3. Ribosomes
4. Vectors

Q.

What are the four stages for DNA preparation from a culture of bacterial cells?

Q. helps joining DNA fragments.

1. Lipase
2. Ligase
3. Pepsin

Q. Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would youfind the smallest segments of DNA ?

1. Near the positive electrode, farthest away from the wells
2. Near the negative electrode, close to the wells
3. Near the top, near the negative pole
4. Near the middle, they tend to slowdown after the first few minutes

Q. What are the products of fermentation

Q.

Which one of the following methods is not a part of traditional biotechnology?

1. Selection and rearing of plants to obtain a superior hybrid variety

2. Using bacteria and fungi to ferment bread

3. Production of insulin through genetic engineering

4. Selection and rearing of cattle to obtain a superior hybrid variety

Q.

In genetic engineering, a vector is used to __________

1. copy a gene

2. produce a gene

3. transfer a gene

4. remove a gene

Q. During fermentation, yeast converts sugars into and bacteria converts starches into .

1. alcohol
2. lactic acid
3. proteins
4. fat

Q.

What is mapping of DNA?

Q.

Which of the following enzyme is called as the 'genetic scissor'?

1. Protease

2. Restriction endonuclease

3. Lipase

4. Ligase

Q.

Explain somatic and germ line gene therapy.

Q.

How does Boron occur in soil?

Q. Darwin's theory of the survival of the fittest states that only the trait with survival advantage will survive.
A . how will you relate this theory in the day to day life?
B . how will you make yourself fit for a particular work?

Q. Transfer of any gene into a completely different organism can be done through

1. tissue culture
2. DNA replication
3. genetic engineering
4. conjugation

Q.

Genetic engineering involves manipulation in

1. DNA

2. RNA

3. Gamete

4. Protein

Q. Information flow or central dogma of molecular biology is __________________.

1. RNA $\to$ Proteins $\to$ DNA
2. Proteins $\to$ RNA $\to$ DNA
3. RNA $\to$ DNA $\to$ Proteins
4. DNA $\to$ RNA $\to$ Proteins

Q. What is a plasmid?

1. Extrachromosomal linear RNA
2. Bacterial, linea, dsDNA
3. Autonomously replicating circular RNA
4. Extrachromosomal circular dsDNA

Q. Biotechnology is employed in different fields of life such as agriculture, medicine etc.

1. True
2. False

Q.

The table below shows qualities of two varieties of coconut.

$$\begin{array}{cccc}Variety& LifeSpan& DiseaseResistance& Yieldin\\ A:ChavakkadOrange& 30-35years& High& 4-5years\\ B:Lakshadweep& 80-100years& Low& 6-8years\end{array}$$

Suppose a new variety is formed by incorporating the gene for disease resistance from Chavakkad orange variety to Lakshadweep variety. What set of characters will the new variety show?

1. Life span: 30-35 years, Disease resistance: high and yield in: 4-5 years

2. Life span:60-70 years, Disease resistance: medium and yield in: 5-7 years

3. Life span:80-100 years, Disease resistance: low and yield in: 6-8 years

4. Life span: 80-100 years, Disease resistance: high and yield in: 6-8 years

Q. Genetic engineering was developed during

1. 1970
2. 1980
3. 1990
4. 2000

Q. The yellow blobs of cells observed by Louis Pasteur in the good fermentation vessels from Bigo's distillery were .

1. bacteria
2. yeast
3. viruses
4. dust