PCR in Diagnosis

Q.

What is the application of PCR in the detection of hereditary diseases?

Q.

The polymerase for a PCR comes from which organism?

1. Homo sapiens

2. ​​​​​Drosophila melanogaster

3. Thermus aquaticus

4. ​​​​​​Staphylococcus aurelia

Q. AIDS can be detected by PCR at an early stage as

1. it carries out isolation fast
2. No other technique is available
3. PCR has to be done before hybridization can be done
4. It amplifies the genetic material

Q. The mRNA from a person is isolated and then a single stranded radiolabelled cDNA is synthesized using mRNA as template (Reverse transcriptase) and the radiolabelled cDNA is then allowed to hybridize with a known strand of DNA.Which one of the following images describes the above experiment?

1. PCR
2. RT - PCR
3. Gel electrophoresis
4. DNA Microarray

Q. Arrange the steps of diagnosing AIDS using the PCR method in the right order:
I - cDNA, engineered primers, and nucleotides along with buffer are incubated in a thermal cycler
II - Primers specific to HIV genes are engineered
III - RNA is extracted from the patient’s plasma
IV - The PCR product is run on a gel to separate and identify the PCR amplified viral genes
V - Reverse transcriptase is used to convert the RNA to cDNA

1. IV, I, V, III, II
2. III, V, II, I, IV
3. V, II, III, IV, I
   
4. I, III, IV, V, II