Somatic Hybridization Process

Q.

Triticale is obtained by

1. Biofortification
2. Cross pollination
3. Somatic hybridization
4. Pollination

Q.
With reference to the above diagram answer the questions that follow.
1 - Name the enzymes
2 - Identify the cells
3 - Name the cell
4 - Identify the particular step in somatic hybridisation
5 - Identify the cell

1. 1 - Cellulase/pectinase, 2 - Protoplasts, 3 - Heterokaryon, 4 - Cell wall regeneration, 5 - Callus
2. 1 - Chitinase/pectinase, 2 - Protoplasts, 3 - Heterokaryon, 4 - Cell wall regeneration, 5 - Callus
3. 1 - Cellulase/protease, 2 - Chloroplasts, 3 - Heterokaryon, 4 - Cell wall regeneration, 5 - Callus
4. 1 - Cellulase/chitinase, 2 - Heterokaryon, 3 - Protoplast, 4 - Callus, 5 - Cell wall regeneration

Q. Somatic hybrids are created using germ cells of desirable parents.

1. False
2. True

Q. Arrange the steps of somatic hybridisation in the correct order.

1. Select hybrids
2. Induce cell wall
3. Degrade cellwall
4. Fuse protoplast
5. Callus