Extraction of Genomic DNA

Q. Chilled ethanol is used in biotechnology for:

1. Creating transient pores in cell membrane
2. DNA precipitation
3. Cell culture preparation
4. Protoplast fusion

Q. Detergent is used to break open the cell membrane. How does detergent aid in this process?

1. Detergent dissolves the cell membrane because of its alkalinity
2. Cell membrane reacts with the acidic detergent and is dissolved in it
3. Detergent breaks the cell membrane after binding to the hydrophobic lipid tails
4. Detergent can make holes in the cell membrane for the DNA to come out

Q. First step in genetic engineering is

1. Isolation of RNA
2. Isolation of protein
3. Isolation of genetic material
4. Purification of protein

Q. ________is added to precipitate purified DNA.

1. Lysozyme
2. Protease
3. Chilled ethanol
4. Cellulase

Q. Match the following cells to their enzymes used for rupturing cells:

1. Lysozyme
2. Cellulase
3. Chitinase