Gel Electrophoresis

Q. The DNA that is cut during restriction enzyme digestion can be separated using __

Q.

Terminalizaiton of chiasma begins during:

1. Zygotene

2. Diplotene

3. Diakinesis

4. Metaphase

Q. Which of the following is true for agarose gel during electrophoresis?

1. The larger the fragment size, the farther it moves
2. The smaller the fragment size, the farther it moves
3. Positively charged fragments move to farther end
4. Negatively charged fragments move to farther end

Q. Agarose extracted from sea wees finds use in:

1. Gel electrophoresis
2. Spectrophotometry
3. Tissue culture
4. PCR

Q. Researchers sometimes use chromatography as a method to separate biomolecules. Why is electrophoresis used and not chromatography for separating DNA molecules?

1. Chromatography is used only for coloured substances
2. Electrophoresis is faster when compared to chromatography
3. DNA is negatively charged and hence can be easily separated using electric charge
4. Chromatography cannot be performed for separating charged molecules

Q. Gel electrophoresis is used for:

1. construction of recombinant DNA by joining with cloning vectors
2. isolation of DNA molecule
3. cutting of DNA into fragments
4. separation of DNA fragments according to their size

Q. Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would youfind the smallest segments of DNA ?

1. Near the positive electrode, farthest away from the wells
2. Near the negative electrode, close to the wells
3. Near the top, near the negative pole
4. Near the middle, they tend to slowdown after the first few minutes