Genetic Engineering
Trending Questions
Q.
How is the luciferase gene isolated?
Q. Transfer of any gene into a completely different organism can be done through
- tissue culture
- DNA replication
- genetic engineering
- conjugation
Q. Multiload device contains____________.
- calcium
- iron
- manganese
- copper
Q.
A technique to produce genetically alike individuals from a single cell is known as ___________.
Q.
What are the tools of DNA technology?
Q. After homogenisation of plant tissue with suitable acid, the retentate fraction was analysed. The compound that will be found in the acid insoluble fraction is:- (1) Cysteine (2) Calcium (3) Cytosine (4) Cellulose
Q. Arrange the given steps involved in recombinant DNA technology in the correct order.
A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme
A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme
- E → C → A → F → B → D
- E → C → A → B → F → D
- A → E → C → B → D → F
- C → E → B → F → A → D
Q.
The technique in which a foreign DNA is coated on the surface of tungsten or gold particles and shot into the target cells is known as
Microinjection
Chemical – mediated gene transfer
- Particle gun
Electroporation
Q.
Which of the following is the correct match of enzymes with their rule in genetic engineering?
DNA polymerase - cutting the DNA
Restriction enzyme - cutting the DNA
DNA ligase - helps in DNA replication-
Restriction enzyme - helps in DNA replication.
Q.
Advancement in genetic engineering has been possible due to
Oncogenes
Exonucleases
Transposons
Endonucleases