Glycolysis Payoff Phase

Q.

Pyruvic acid, the key product of glycolysis, can have many metabolic fates. Under aerobic conditions, it forms

1. CO₂ + H₂O

2. Lactic acid

3. CO₂

4. Acetyl CoA + CO₂

Q. During glycolysis, the final step is the conversion of phosphoenolpyruvate to pyruvate with the help of an enzyme. Can you pick which of the following curves is with and without the presence of enzyme?

1. Endothermic reaction with energy A in absence of enzyme and B in the presence of enzyme
2. Exothermic reaction with energy A in absence of enzyme and B in the presence of enzyme
3. Endothermic reaction with energy A in presence of enzyme and B in the absence of enzyme
4. Exothermic reaction with energy A in presence of enzyme and B in the absence of enzyme

Q.

Is ADP a product of glycolysis?

Q. Identify the missing compounds:

1. A - Phosphoenolpyruvate,
   B - Fructose-1, 6-phosphate
2. A - Glucose-6-phosphate,
   B - Phosphoglyceric acid
3. A - Dihydroxy acetone phosphate,
   B - 3-phosphoglyceric acid
4. A - Triose phosphate,
   B - Phosphoenolpyruvate

Q. Choose the steps of glycolysis where substrate level phosphorylation occurs:
(a) Glucose $\to$ Glucose-6-phosphate
(b) $1,3$-bisphosphoglyceric acid $\to$ 3-phosphoglyceric acid
(c) $1,3$-bisphosphoglyceraldehyde $\to$ $1,3,$-bisphosphoglyceric acid
(d) Phosphoenolpyruvate $\to$ Pyruvate
(e) Fructose-6-phosphate $\to$ Fructose-$1,6$-bisphosphate

1. a, e
2. a, c
3. b, d
4. a, b, d, e

Q. The total number of substrate level phosphorylation reactions that occur during conversion of 1 glucose into two pyruvic acids is

1. 4
2. 1
3. 8
4. 2

Q. In which of the following reactions of glycolysis does oxidation take place?

1. Glucose-6-phosphate to fructose-6-phosphate
2. Glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate
3. 1, 3-Diphosphoglycerate to 3-phosphoglycerate
4. 2-Diphosphoglycerate to phosphoglycerate

Q. During oxidation in glycolysis, electrons are removed from

1. $\text{ATP}$
2. $\text{glyceraldehyde-3-phosphate}$
3. ${\text{NAD}}^{+}$
4. $\text{molecular oxygen}$

Q.

NADPH is generated through

1. Glycolysis

2. Aerobic respiration

3. PS II

4. PS I

Q. Match the following:

$\begin{array}{cc}\text{i. Invertase}& \text{1. Glucose}\\ \text{ii. Hexokinase}& \text{2. 2-phosphoglycerate}\\ \text{iii. Enolase}& \text{3. Sucrose}\end{array}$

1. i-2, ii-3, iii-1
2. i-1, ii-3, iii-2
3. i-2, ii-1, iii-3
4. i-3, ii-1, iii-2

Q. Direct ATP synthesis in glycolysis occurs during conversion of ____i____ and ____ii____ into ____iii____ and ____iv____ respectively.

1. i: Fructose-6-phosphate, ii: Glucose-6-phosphate, iii: Fructose-1, 6-biphosphate, iv: 2-phosphoglycerate
2. i: Glucose-6-phosphate, ii: Fructose-6-phosphate, iii: Fructose-1, 6-biphosphate, iv: 3-phosphoglyceric acid
3. i: 1, 3-bisphosphoglycerate, ii: Phosphoenolpyruvate, iii: 3-phosphoglycerate, iv: Pyruvate
4. i: 1, 3-bisphosphoglycerate, ii: 3-phosphoglycerate, iii: Phosphoenolpyruvate, iv: 2-phosphoglyceric acid

Q. How many NADH + $H^{+}$ molecules are formed if four glucose molecules enter glycolysis?

1. 2
2. 4
3. 8
4. 16

Q. Which one of the following does not involve isomerisation in glycolysis pathway?

1. Conversion of glucose-6-phosphate to fructose-6-phosphate
2. Conversion of glycerol aldehyde-3-phosphate to dihydroxyacetone phosphate
3. Conversion of 3-phosphoglyceric acid to 2-phosphoglyceric acid
4. Conversion of 2-phosphoglyceric acid to phospho enol pyruvic acid

Q. Match the following molecules released when the products (in the right column) are formed during glycolysis:

$\begin{array}{cc}\text{i. ATP}& \text{1. Triose bisphosphate}\\ \text{ii. NADH}& \text{2. Phosphoenolpyruvate}\\ \text{iii.}{H}_{2}O& \text{3. Triose phosphate}\end{array}$

1. i-2, ii-3, iii-1
2. i-3, ii-1, iii-2
3. i-1, ii-3, iii-2
4. i-1, ii-2, iii-3

Q.

The proper sequence of stages in glycolysis is

1. Glucose priming, cleavage and rearrangement, oxidation, ATP generation

2. Cleavage and rearrangement, glucose priming, ATP generation, oxidation

3. Glucose priming, oxidation, cleavage and rearrangement, ATP generation

4. ATP generation, oxidation, glucose priming, cleavage and rearrangement