Obtaining Desired Gene

Q. Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli

1. ii-i-iv-iii-v-vi
2. i-iii-v-vi-ii-iv
3. i-iv-v-ii-vi-iii
4. iii-v-ii-i-vi-iv

Q. The sequence complementary to the sequence of the spliced and mature mRNA is .

1. rDNA
2. cDNA
3. genomic DNA
4. protein sequence

Q. The non protein coding regions in DNA are known as whereas the protein coding regions are known as .

1. exons
2. introns
3. genes
4. cDNA