Qualities of a Good Vector

Q.

What are the characteristics of cloning vectors?

Q.

Why is pBR322 a good cloning vector?

Q. The ability to replicate is not a necessary feature of a vector.

1. False
2. True

Q. In 1928, Frederick Griffith showed that the exogenous hereditary material can be made to enter into a bacteria through a process called

1. transformation
2. translation
3. transduction
4. transcription

Q. A scientist synthesised a new plasmid for her research work. Her intention was to insert a human gene into the plasmid and harvest large amounts of protein. But in spite of several rounds of experimentation, she found that these bacteria did not produce the protein of choice. What could be the problem with the plasmid?

1. Transgene is not inserted in the correct position in plasmid
2. The plasmid does not have the transgene
3. The plasmid does not have a marker
4. The ori site is missing

Q. Markers are usually antibiotic __ genes.

Q. Which of the following key factors makes the plasmid a good vector in genetic engineering?

1. Plasmid has an Ori sequence
2. It is self replicating
3. It has ability to carry a foreign gene
4. Its ability to cause infection

Q. What is the importance of chromosome replication in interphase?

Q. A plasmid vector should have

1. origin of replication.
2. selectable marker.
3. cloning site.
4. high copy number.