Restriction Enzyme

Q. The specific DNA sequence where Eco – RI cuts is

1. GATTCG
2. GAATTC
3. GTTCAA
4. TTCCAA

Q.

Write short notes on:

Phagocytosis

Q. Restriction enzymes present in micro-organisms do not cut their own DNA because of the presence of the group that blocks digestion.

1. methyl
2. ethyl
3. butyl
4. aromatic

Q. Endonuclease is used in

1. Genetic engineering
2. Transcription
3. Translation
4. None of these

Q. Match column I with their correct match in column II.

The correct match is

Q. Which one is regarded as a molecular scissor in biotechnology?

1. Reverse transcriptase
2. Restriction endonuclease
3. Taq polymerase
4. Topoisomerase

Q. Eco RI is

1. terminal transferase
2. restriction enzyme
3. vector plasmid
4. vector virus

Q. Restriction sites are pallindromic in nature.

1. True
2. False

Q. A conventional method for naming restriction enzymes is followed.In case of EcoRI, what do the following indicate

1. 1 - Strain 2 - Genus3 - Order of isolation 4 - Species
2. 1 - Genus 2 - Order of isolation 3 - Strain 4 - Species
3. 1 - Genus 2 - Species 3 - Strain 4 - Order of isolation
4. 1 - Order of isolation 2 - Species 3 - Strain 4 - Genus

Q. Which of the following is specifically used in genetic engineering?

1. Ligase
2. Gyrase
3. Restriction endonuclease
4. DNA polymerase

Q. GAATTC is the reorganization site for which of the following restriction endonuclease?

1. Hin d III
2. Eco R I
3. Bam I
4. Hae III

Q. The bonds which provide stability to the structure of myoglobin are
(A) Hydrogen bonds
(B) van der Waals interaction
(C) Phosphodiester bonds
(D) Hydrophobic interactions
(E) Electrostatic interactions
Choose the option which includes all the correct ones.

Q. In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to

1. Isolate fragments of cloned bacterial plasmids.
2. Isolate fragments of frog DNA that contained an rRNA gene.
3. Cleave the bacterial plasmid.
4. All the above

Q. In the resistriction endonuclease EcoRI, what does ‘co’ stand for?

1. Coelom
2. Coenzyme
3. Coli
4. Colon

Q. Which of the following was a major contribution of Stanley Cohen in creating the first recombinant DNA molecule?

1. Developing a method of removing the plasmids from one cell and then reinserting them in other cells
2. All of these
3. Combining a gene from a plant with a plasmid of Escherichia coli
4. Discovery of reverse transcriptase

Q. Bacteria use restriction enzymes for _______ .

1. cutting its own DNA as a defense mechanism
2. cutting DNA of other bacteria
3. cutting DNA of viruses that invade them
4. cutting its own DNA during replication

Q. Scientists isolated a restriction enzyme from Arthrobacter luteus for the first time and need to name it. Which of the following names do you think should be used?

1. RalII
2. ArlI
3. AluI
4. ResI

Q. A graduate student working on a project is attempting to isolate a gene from yeast. He identifies the restriction sites to cut the gene of interest out. But after several attempts, fails to do so. What could be the possible reason for the failure?

1. Yeast genome is too complex containing overlapping genes
2. Restriction enzyme used has recognition sites in the gene
3. The gene of interest is too big to isolate
4. The gene of interest in yeast is methylated