Screening of Recombinant Cell

Q. Read the given statements and select the correct option.
Statement-1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement-2: Presence of insert results into insertional inactivation of beta- galactosidase enzyme and the colonies do not produce any colour.

1. Both statements 1 and 2 are correct and statement 2 is correct explanation of statement 1
2. Both statements 1 and 2 are correct but statement 2 is not correct explanation of statement 1
3. Statement 1 is correct and statement 2 is incorrect
4. Both statements 1 and 2 are incorrect

Q.

Which of the following is true about insertional inactivation of the β-galactosidase coding sequence?

1. The transformants exhibit blue coloured

2. The non-transformants exhibit blue coloured

3. The transformants do not exhibit any colour

4. The Non-transformants do not exhibit any colour

Q. How many of the following statement(s) regarding insertional inactivation of lacZ gene are incorrect?

1. lacZ gene remains active in non-recombinant transformed bacteria.
2. X-gal gets hydrolysed and produces a blue product in non-recombinant transformed bacteria.
3. Bacterial colonies which are transformed with recombinant DNA appear white.
4. 𝛃-Galactosidase is produced in recombinant bacteria.
5. Bacterial colonies which are transformed with recombinant DNA appear blue.

1. Four
2. Two
3. One
4. Three

Q. Identify the option with the correct match for I, II and III.

1. I - Biolistics/ gene gun, II - Transformation of animal cells, III - Direct method of gene delivery
2. I - EcoR I, II - Restriction enzyme, III - Blunt ends
3. I - YAC, II - Plasmid, III - Obtained from bacteria
4. I - lacZ, II - Selectable marker, III - Selects recombinants