What is PCR

Q. The polymerase Chain Reaction (PCR) is technique that is used for

1. in vivo replication of specific DNA sequence using thermostable DNA polymerase
2. in vitro synthesis of mRNA
3. in vitro replication of specific DNA sequence using thermostable DNA polymerase
4. in vivo synthesis of mRNA
5. separation of DNA fragments according to their size

Q.

what are the components of nucleic acid?

Q. In vitro clonal propagation in plants in characterized by:

1. PCR and RAPD
2. Northern blotting
3. Electrophoresis and HPLC
4. Microscopy

Q. Assertion: Small circular DNA in bacterial cell are plasmids.Reason: Plasmids confers certain unique characters of bacteria

Q. The Polymerase chain reaction (PCR) is a powerful technique to

1. Mutate Genes
2. Amplify genes
3. Inhibit DNA synthesis
4. Induce protein synthesis

Q. Which of the following polymerases are used in PCR?

1. DNA polymerase I
2. DNA polymerase I
3. DNA polymerase III
4. Taq polymerase

Q. Match the organism with its use in biotechnology.

a	Bacillus thuringiensis	i	Cloning vector
b	Thermus aquaticus	ii	Construction of first rDNA molecule
c	Agrobacterium tumefaciens	iii	DNA polymerase
d	Salmonella typhimurium	iv	Cry proteins

Select the correct option from the following

1. a - ii, b - iv, c - iii, d - i
2. a - iii, b - ii, c - iv, d - i
3. a - iv, b - iii, c - i, d - ii
4. a - iii, b - iv, c - i, d - ii

Q. Palaeontologists unearthed a human skull during excavation. A small fragment of the scalp tissue was still attached to it. Only a little DNA could be extracted from it. If the genes of the ancient man need to be analysed, the best way of getting sufficient amount of DNA from this extract is by

1. hybridising the DNA with a DNA probe
2. subjecting the DNA to polymerase chain reaction
3. subjecting the DNA to gel electrophoresis
4. treating the DNA with restriction endonucleases

Q. PCR amplifies DNA strands in an exponential rate.

1. False
2. True

Q. The copies made by PCR are not identical and have certain variations.

1. True
2. False

Q.

PCR is required for

1. DNA synthesis

2. DNA amplification

3. Protein synthesis

4. Amino acid synthesis

Q. Which of the following statements is true regarding the DNA polymerase used in PCR?

1. It is isolated from a virus
2. It is used to ligate/join introduced DNA in recipient cells
3. It remains active at high temperatures
4. It serves as a DNA digesting enzyme