BAC and YAC

Q. Which of following feature is not necessary for cloning vector?

1. Origin of replication
2. High copy number
3. Selectable marker
4. Cloning sites

Q.

What would happen if a plasmid without a selectable marker was chosen as a cloning vector?

Q. Choose the correct option(s) regarding BAC used in Human Genome project (HGP)

1. BAC is the only vector used in HGP
2. Human genome can be cut into size up to 200, 000 base pairs and can be inserted in BACs
3. BAC stands for beneficial artificial chromosome
4. All of the above

Q. Which is the vector suitable for cloning long DNA fragments ?(A)Yeast plasmid
(B)cosmids

Q. Assertion :Repetitive sequences make up very large portion of human genome. Reason: Repetitive sequences do not have direct coding functions in the genome.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Which of the following is incorrect statement about the plasmids?

1. It is the extra chromosomal DNA in bacteria
2. None of the above
3. It is not a integral part, but inert genetic material
4. Host chromosomes can be integrated with the plasmid

Q. Commonly used vectors for human genome sequencing are

1. T-DNA
2. BAC and YAC
3. Expression vectors
4. T/A cloning vectors

Q. Commonly used vectors for human genome sequencing are ?

1. T-DNA
2. BAC and YAC
3. expression vectors
4. T/A cloning vectors

Q. The extra-chromosomal DNA found in the bacterium E.coli is called _________.

1. Mesosome
2. Nucleoid
3. Incipient nucleus
4. Plasmid

Q. The plasmid called recombinant plasmid and used for introduction of large genome to host cells i ___________.

1. Lamda
2. R plasmid
3. Cosmid
4. F plasmid

Q. Choose the vector which is used in gene mapping.

1. Phage vector
2. Cosmid vector
3. Yeast artificial chromosome (YAC)
4. Plasmid vector

Q. The gene promoter sequences, commonly used in gene cloning experiments are derived from __________________.

1. TMV(Tobacco Mosaic Virus)
2. CaMV(Cauliflower Mosaic Virus)
3. CMV(Cucumber Mosaic Virus)
4. TSWV(Tomato Spotted Wilt Virus)

Q. A scientist isolated his gene of interest and found it to be 660 kb in size. Which of the following vectors should he use to transfer the gene of interest?

1. Plasmid
2. BAC
3. YAC
4. Cosmid

Q. Some genes in bacteria and virus may code for more than one polypeptide. They are __________ .

1. Split genes
2. Overlapping genes
3. Jumping genes
4. None of the above

Q. Choose the vector which is used in gene mapping.

1. Plasmid vector
2. Phage vector
3. Cosmid vector
4. Yeast artificial chromosome (YAC)

Q. Commonly used vectors for human genome sequencing are

1. T-DNA
2. T/A cloning vectors
3. BAC and YAC
4. Expression vectors

Q.

vector DNA is double stranded or single stranded ?

rn rn

Q. The term 'transposon' for jumping genes is coined by

1. Lederberg
2. Zinder and Lederberg
3. Hedges and Jacob
4. Jenssen