CDNA Technology

Q.

Which of the following RNAs is not required for the synthesis of protein?

1. siRNA

2. tRNA

3. rRNA

4. mRNA

Q. The process of making multiple identical copies of any template DNA is referred to as ________.

1. gene cloning
2. restriction digestion
3. gene transfer
4. bioprocess engineering

Q.

Draw and label diagram of tRNA.

Q.

Differentiate between Transcription and Translation?

Q. Which of the following occurs during translation?

1. Formation of hnRNA
2. Splicing
3. Ribosome binds to the mRNA
4. Capping

Q. What does "lac" refer to in the lac operon?

1. Lactose
2. Lactase
3. Lac insect
4. The number 1, 00, 000

Q.

Is cDNA the same as mRNA?

Q. The lac operon consists of:

1. One regulatory gene and three structural genes (z, y, a)
2. Three regulatory genes and one structural gene
3. One regulatory gene and many structural genes
4. One regulatory gene and one structural gene

Q. Match the following genes of the Lac operon with their respective products.

Column I	Column II
A. i gene	i. beta-galactosidase
B. z gene	ii. Permease
C. a gene	iii. Repressor
D. y gene	iv. Transacetylase

1. A-i, B- iii, C- ii, D- iv
2. A- iii, B-i, C-ii, D-iv
3. A-iii, B- iv, C- i, D- ii
4. A-iii, B-i, C- iv, D-ii

Q. c-DNA is synthesized using a template of

1. DNA
2. m-RNA
3. r-RNA
4. t-RNA

Q. What is the major difference between DNA and cDNA?

1. Exons absent in DNA
2. Both (A) and (B)
3. Introns absent in cDNA
4. Introns present in cDNA

Q. In genetic coding, triplet codons make possible 64 types of codons. If the genetic code was a doublet instead of a triplet codon, the number of types of codons possible would be

1. 64 types of codons
2. 4 types of codons
3. 24 types of codons
4. 16 types of codons

Q. How many nucleotides are found in one spiral of B-DNA?

1. 5
2. 10
3. 20
4. 25

Q. cDNA sequence is complementary to

1. Gene sequence in genomic DNA
2. Sequence in transcribed mRNA
3. Sequence in spliced and mature mRNA
4. Protein sequence

Q. c-DNA is synthesized using a template of

1. DNA
2. m-RNA
3. r-RNA
4. t-RNA

Q. 64 Codons constitute genetic code because

1. There was 64 types of amino acid.
2. 64 types of tRNA.
3. Genetic code is triplet.
4. There are 64 enzymes.

Q. Match the following genes of the Lac operon with their respective products.

Column I	Column II
A. i gene	i. beta-galactosidase
B. z gene	ii. Permease
C. a gene	iii. Repressor
D. y gene	iv. Transacetylase

1. A-iii, B- iv, C- i, D- ii
2. A- iii, B-i, C-ii, D-iv
3. A-i, B- iii, C- ii, D- iv
4. A-iii, B-i, C- iv, D-ii

Q. Match the following genes of the Lac operon with their respective products.

Column I	Column II
A. i gene	i. beta-galactosidase
B. z gene	ii. Permease
C. a gene	iii. Repressor
D. y gene	iv. Transacetylase

1. A-iii, B-i, C- iv, D-ii
2. A-iii, B- iv, C- i, D- ii
3. A-i, B- iii, C- ii, D- iv
4. A- iii, B-i, C-ii, D-iv

Q.

DNA polymerase helps in ________________.

1. Renaturation
2. Denaturation
3. Splitting or separation of two strands of DNA
4. Joining bits of DNA

Q.

cDNA is always:

1. Single-stranded and linear
   

2. Double-stranded and linear

3. Double-stranded and circular

4. None of the above

Q. cDNA sequence is complementary to

1. Gene sequence in genomic DNA
2. Sequence in transcribed mRNA
3. Sequence in spliced and mature mRNA
4. Protein sequence

Q. The type of RNA that carries information for the sequence of amino acids during protein synthesis is ______________.

Q.

cDNA is always:

1. Double-stranded and linear

2. Double-stranded and circular

3. Single-stranded and linear
   

4. None of the above

Q. c-DNA probes are copied from the mRNA molecules with the help of

1. reverse transcriptase
2. restriction enzymes
3. DNA polymerase
4. adenosine deaminase

Q. cDNA sequence is complementary to

1. Gene sequence in genomic DNA
2. Sequence in transcribed mRNA
3. Sequence in spliced and mature mRNA
4. Protein sequence