Cosmid Vector
Trending Questions
Q. Plasmid vector in DNA recombinant technology means
- extra-chromosomal autonomously replicating circular DNA
- any fragment of DNA carrying a desirable gene
- a virus that transfers gene to bacteria
- sticky end of DNA
Q.
What are the 2 most commonly used vectors?
Q. A cosmid is a
- naturally occurring vector
- vector constructed using plasmid DNA and cos site of Lambda phage DNA
- plasmid DNA with RNA
- none of the above
Q. The most important feature present in a plasmid so it can be used as a vector is
- origin of replication
- presence of selectable marker
- presence of sites for restriction endonuclease
- presence of alternate selectable marker
Q.
What are the types of plasmids in bacterial cells?
Q. Match the vectors with their foreign DNA insert size:
- 200-2000 kb
- 100-300 kb
- 35-45 kb
- upto 7 kb
Q. Biolistic gun is suitable for
- DNA fingerprinting
- Disarming pathogen vectors
- Transformation of plant cells
- Constructing recombinant DNA
Q.
In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector.
Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
(i) Bacterium
(ii) Plasmid
(iii) Plasmodium
(iv) Bacteriophage
Q. Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Q. Somaclones are ________________.
- Genetically identical to the original plant
- Used to recover disease free plants
- Somatic hybrids
- Sterile plants
Q. The extra chromosomal genetic material seen in a bacterial cell capable of replication is called as
- Plasmid
- Mesome
- Nucleoid
- Chromatin
Q. A piece of nucleic acid used to find, a gene by forming a hybrid with it is called a
- probe
- retrovirus
- vector
- restriction sequence
Q. An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can ______________.
- Reproduce more rapidly
- Excise introns from the RNA transcript
- Remove methyl groups
- Produce restriction enzymes
Q. Match the vectors with their foreign DNA insert size:
- 200-2000 kb
- 100-300 kb
- 35-45 kb
- upto 7 kb
Q. Cosmid is
- Circular extranuclear DNA of bacteria
- Extragenetic material in mycoplasma
- Fragment of genetic material inserted in cells to replace defective genes
- Fragment of DNA inserted in bacteria for forming its copies
Q. Hirudin gene inserted in Brassica napus was?
- Synthesised chemically
- Obtained from leech
- Got from Bacillus thuringiensis
- Brassica campestris
Q. Which of the following statement is/are incorrect?
- Cosmids contain gene coding for viral proteins.
- Cosmids replicate like plasmids.
- They have cos sites with 12 nucleotide bases which prevent them from circularising.
- Cosmids have antibiotic resistance marker genes.
Q. The enzyme, DNA polymerase was discovered by
- Kornberg
- Okazaki
- Waston and Crick
- Jacob and Monod
Q. Which of the following is not used to transfer the recombinant DNA into the host?
- Gene gun method
- Micro-injection method
- Bioreactors
- Disarmed pathogen vectors
Q. Transfer of DNA from one bacteria to another by contact is known as
- Conjugation
- Transformation
- Transduction
- Transcription
Q. Which is the specific DNA sequence where Eco R1 cuts?
- GAATTC CTTAAG
- ATTCGATAAGCT
- GTTCAA CAAGTT
- GCTTAA CGAATT
Q. Cosmid is
- Fragment of DNA inserted in bacteria for forming copies
- Extra DNA in bacteria
- Circular DNA in bacteria
- Extragenetic material In Mycoplasma
Q. Difference between YAC and BAC
Q. Genetic code was discovered by
- Nirenberg & Matthaei
- Kornberg & Crick
- Khorana & Kornberg
- Gamow
Q. A cosmid is a
- naturally occurring vector
- vector constructed using plasmid DNA and cos site of Lambda phage DNA
- plasmid DNA with RNA
- none of the above
Q. Kappa particles were discovered by
- Sonneborn
- Rhoades
- Bycott et al.
- Correns
