DNA Polymerase

Q. DNA dependent RNA polymerase catalyzes transcription on the strand of the DNA which is called the

1. alpha strand
2. antistrand
3. template strand
4. coding strand

Q.

DNA Polymerase adds nucleotides to the free ___ end of RNA primers.

Q.

Depending upon the chemical nature of the template (DNA or RNA) and the nature of nucleic acids synthesised from it (DNA or RNA), list the types of nucleic acid polymerases.

Q. In DNA replication, the primer is

1. Small deoxyribonucleotide polymer
2. Enzyme taking part in joining nucleotides to their complementary template bases.
3. Small ribonucleotide polymer
4. Helix destabilising protein

Q.

Is ori a selectable marker?

Q.

What triggers DNA replication?

Q. Eco RI and Rsa I restriction endonucleases require $6$ and $4$ bp sequences respectively for cleavage. In a $10kb$ DNA fragment how many probable cleavage sites are present for these enzymes

1. $0$ Eco RI and $10$ Rsa I
2. $1$ Eco RI and $29$ Rsa I
3. $4$ Eco RI and $69$ Rsa I
4. $2$ Eco RI and $39$ Rsa I

Q. During DNA replication, Okazaki fragments are used to elongate

1. the leading strand away from replication fork
2. the lagging strand towards replication fork
3. the lagging strand away from the replication fork
4. the leading strand towards replication fork

Q. Transcription involves synthesis of............ from a DNA molecule.

1. DNA molecule
2. RNA molecule
3. Protein
4. Both DNA and RNA

Q. In one polynucleotide strand of a $DNA$ molecule the ratio of $A+T/G+C$ is $0.3$. What is the $A+G/T+C$ ratio of the entire $DNA$ molecule?

Q.

Which of the following properites of DNA polymerise necessitates the requirement of RNA primer in DNA replication?

1. It gets denatured at high temperatures

2. It can add a nucleotide only to a 5' end

3. It can add a nucleotide only to a 3' end

4. None of the above

Q. DNA polymerase enzyme is required for the synthesis of

1. RNA from DNA
2. DNA from DNA
3. RNA from RNA
4. DNA from RNA

Q.

DNA polymerase adds nucleotides to the free ___ end of RNA primers.

Q. All of the following statements characterize DNA replication except

1. Second DNA strand is in form of Okazaki segments.
2. DNA is synthesized in 3' to 5' direction in one strand and 5' to 3' in another.
3. Growth of one DNA chain is discontinuous.
4. Overall growth of DNA chain is bidirectional.

Q. Why is the Strand of DNA of polarity 3-5 generated discontinuosly during DNA Replication?

Q. What is the direction of synthesis of DNA by DNA Polymerase?

1. 3' to 5'
2. 5' to 3'
3. No set direction for DNA Polymerase
4. Direction is decided by the Ori sequence

Q. What is the direction of synthesis of DNA by DNA Polymerase?

1. 3' to 5'
2. 5' to 3'
3. No set direction for DNA Polymerase
4. Direction is decided by the Ori sequence

Q.

DNA polymerase adds nucleotides to the free ___ end of RNA primers.

Q. can we take 3 to 5 strand as a coding strand in transcription unit

Q. Why are nucleotides added to $3^{\prime }$ end?

Q. Observe the table in which size of different DNA fragments are given and answer the questions:

DNA fragments	$A$	$B$	$C$
Size (in Base Pairs)	$700$	$1500$	$3000$

In the process of separating DNA fragments which fragements moves faster?

Q.

Depending upon the chemical nature of the template (DNA or RNA) and the nature of nucleic acids synthesised from it (DNA or RNA), list the types of nucleic acid polymerases.

Q.

The number of tins of paint solved by three different shops is given below

6

Draw the pictograph using scale, represents $20$ tins and answer how many figures must be drawn for shop $C$, If the total number of tins sold is $400$

Q.

The number of tins of paint solved by three different shops is given below

Draw the pictograph by taking scale, represents $20$ tins and find the difference between tins sold by shop $\mathrm{B}$ and tins sold by shop $C$.

Q. If the base sequence of one DNA template strand is CAACATGTAGCAAGT, what would be the base sequence in the RNA transcribed against it?

Q. DNA dependent RNA polymerase catalyzes transcription on the strand of the DNA which is called the

1. antistrand
2. template strand
3. coding strand
4. alpha strand

Q. What is the direction of synthesis of DNA by DNA Polymerase?

1. 3' to 5'
2. 5' to 3'
3. No set direction for DNA Polymerase
4. Direction is decided by the Ori sequence