Desired Gene Identification

Q. Spooling is:

1. Amplification of DNA
2. Cutting of separated DNA bands from the agarose gel
3. Transfer of separated DNA fragments to synthetic membranes
4. Collection of isolated DNA

Q. A gene of interest (GOI), amplified using PCR, can be isolated using agarose gel electrophoresis by ____i____ and the DNA is extracted out. This process is known as ____ii____.

1. i - cutting the portion of gel of the desired band, ii - elution of DNA
2. i - scraping the gel of the desired band, ii - extraction of DNA
3. i - extracting the DNA from the entire gel, ii - digestion of DNA with restriction enzymes
4. i - cutting the gel of the desired band, ii - amplification of DNA

Q.

How is subtractive hybridization used in comparing gene expressions?

Q. Maize hybrids developed by biofortification had more amount of _________than the existing maize hybrids.

1. Vitamins
2. Minerals
3. Amino acids
4. oils

Q. PCR and Restriction Fragment Length Polymorphism are the methods used for which of the following techniques?

1. Study of enzymes
2. DNA sequencing
3. Genetic transformation
4. Genetic fingerprinting

Q. Restriction fragment length polymorphism (RFLP) analysis is an important tool in

1. Paternity testing
2. Genome mapping
3. All of the above
4. Localizing genes for genetic disorders

Q. The restriction enzymes are used in genetic engineering because they

1. Can degrade harmful proteins
2. Can cut DNA at specific base sequence
3. Can join different DNA fragments
4. Are nucleases that cut RNA at variable sites

Q. The ability to change amino acid sequence of a protein by altering the sequence of its cDNA is called as

1. Genetic engineering
2. Protein engineering
3. Finger printing
4. DNA sequencing

Q. Identify the incorrect statement representing the feature of restriction fragment length polymorphism.

1. They can be used to detect single gene mutations in human DNA.
2. They can be used prenatally to analyze fetal cells for genetic disorders.
3. They can be used to amplify large quantities of a single gene through use of the polymerase chain reaction.
4. They can be used to detect variation in DNA sequences among individuals.
5. They can be separated by electrophoresis according to fragment size.

Q. During gene amplification the mixture of sample DNA, nucleotides and polymerases is heated to

1. Separate sense and antisense DNA strands
2. Activate polymerases
3. Activate nucleotides
4. Provide energy for different strands

Q. Dwarf pea & Maize test are bio-assay of:-

1. Auxin
2. Gibberellins
3. Cytokinins
4. ABA

Q. An analysis of chromosomal DNA using the Southern hybridization technique does not use:

1. Autoradiography
2. PCR
3. Electrophoresis
4. Blotting

Q. Restriction endonuclease, in DNA fingerprinting, carries out the following process.

1. Getting copies of DNA
2. Loading DNA on agaroseplate
3. Fragmentation of DNA
4. Synthesis of DNA

Q. Which among the following is the first step in the R-DNA technology process?

1. Extraction of desired gene using Restriction Endonuclease
2. Amplification of gene by PCR
3. Using vector to clone the desired gene
4. Identification of desired gene by mapping the nucleotide sequence