Discovery of Restriction Enzyme

Q.

What are the restriction sites in pBR322?

Q.

What is the function of restriction endonuclease?

Q.

I need an short explanation about Lysogenic cycle

Q. Among the following, select the tools of recombinant DNA technology
a. Restriction enzymes
b. Polymerase enzymes
c. Ligases
d. Vectors
e. Host organisms

1. a, b, c, d and e
2. a, c, d and e
3. a, b, c and d
4. a, b, c and e

Q.

Which type of restriction enzymes are used in recombinant DNA technology?

Q. The first recombinant DNA was constructed by linking an antibiotic-resistant gene with the native plasmid of

1. Escherichia coli
2. Salmonella typhimurium
3. Clostridium butylicum
4. Acetobacter aceti

Q.

Why is restriction endonuclease so called?

Q. Restriction endonucleases are

1. Present in mammalian cells for degradation of DNA when the cell dies
2. Used in genetic engineering for ligating two DNA molecules
3. Used for in vitro DNA synthesis
4. Synthesized by bacteria as part of their defence mechanism

Q. Following are the applications of PCR, except:
I. Detection of the transgene in an organism
II. DNA fingerprinting
III. Diagnosis of specific mutation
IV. Sequencing of a gene of interest

Choose the correct option

1. III only
2. IV only
3. II and III
4. II only

Q.

Molecular scissor used in genetic engineering is ________.

Q. Which of the following features are required to facilitate cloning into a vector?
a. Origin of replication (ori)
b. Selectable marker
c. Cloning site

1. a and c
2. b and c
3. a and b
4. a, b and c

Q. There is a restriction endonuclease called EcoRI. What does ‘co’ part in it stand for?

1. coli
2. Coelom
3. Colon
4. Coenzyme

Q. Assertion: A gene from Bacillus thuringiensis is inserted into the plant genome to make it pest resistant.
Reason: Bacillus thuringiensis produce Cry protein during the sporulation phase of their growth.

1. Both assertion and reason are true and the reason is a correct explanation of the assertion
2. Both assertion and reason are true but the reason is not the correct explanation of the assertion
   
3. The assertion is true but the reason is false
   
4. Both the assertion and reason are false
   

Q. Which of the following conditions is not possible to accomplish with the plasmid pBR322?

1. Insertional inactivation of ampicillin resistant gene
2. Insertional inactivation of tetracycline resistant gene
3. Insertional inactivation of rop gene
4. Insertional inactivation of ori

Q.

Why is it important that the pieces of DNA are cut with the same restriction enzymes?

Q. What are Lytic and Lysogenic cycle in Phagic type of reproduction of viruses?

Q. Which among the following options regarding protein synthesis is/are correct?

1. Uncharged amino acids are picked up by tRNA molecules randomly from the cytoplasm and are incorporated into the peptide
2. Unprocessed mRNA is translated into the peptide in eukaryotes
3. Most of the aminoacyl tRNA synthetase contains editing sites in addition to the acylation site
4. Proteins are synthesised in the nucleus and then transported to the cytoplasm for their processing

Q.

What type of restriction endonuclease is EcoRI?

Q. Restriction endonuclease Hind II always cuts DNA molecule at a particular point by recognising a specific sequence of

1. Six base pairs
2. Five base pairs
3. Four base pairs
4. Seven base pairs
5. Three base pairs

Q. Separation and purification by filtration, centrifugation of culture broth are a part of

1. Upstream and downstream processing
2. Downstream processing
3. Upstream processing
4. Screening for recombinants and downstream processing

Q. Restriction enzyme was discovered by

1. Waksman
2. Alexander Fleming
3. Berg
4. A. Werner, H. Smith and Nathans

Q.

Does the plasmid using as a vector should be of same type of host?

Q. Restriction endonucleases are

1. Present in mammalian cells for degradation of DNA when the cell dies
2. Used in genetic engineering for ligating two DNA molecules
3. Used for in vitro DNA synthesis
4. Synthesized by bacteria as part of their defence mechanism

Q. The name of Temin and Baltimore is associated with

1. Photorespiration
2. Reverse transcription
3. RNA synthesis
4. All of the above

Q. Exceptions in plant virus having DNA?

Q. The first restriction endonuclease isolated was

1. EcoRI
2. BamHI
3. SalI
4. Hindll

Q. Restriction endonucleases are

1. Present in mammalian cells for degradation of DNA when the cell dies
2. Used in genetic engineering for ligating two DNA molecules
3. Used for in vitro DNA synthesis
4. Synthesized by bacteria as part of their defence mechanism

Q. Restriction enzymes are considered as a type of endonuclease. Why?

Q. Which of the following restriction endonuclease was discovered first?

1. Hind II
2. Bam HI
3. EcoR II
4. EcoRI

Q. Which of the following contains the key tools for recombinant DNA technology?
(i) Restriction endonucleases, ligases, vectors(ii) Ligases, host organism, polymerase enzymes(iii) Vectors, Taq polymerase, primers(iv) Restriction exonucleases, ligases, primers, bioreactors

1. (i) and (ii)
2. (i), (iii) and (iv)
3. (i), (ii) and (iii)
4. (iii) and (iv)