Disintegration of Cell

Q. Which of the following is not a component of downstream processing?

1. Preservation
2. Expression
3. Separation
4. Purification

Q. Cell disintegration during downstream processing is

1. Stirring cells to prevent clumping for better growth
2. Rupturing cell walls and/or cell membranes to release product of interest formed within the cell
3. Clumping cells using chemicals for better separation
4. None of the above

Q. How do substance like co2 and water move in and out of a cell

Q. Assertion: Disintegration of cells is performed using chemical, physical and enzymatic methods.
Reason: Product of interest must not be damaged during cell disintegration.

1. [A] is true and [R] is false.
2. Both [A] and [R] are true and [R] is a correct explanation to [A].
3. Both [A] and [R] are true but [R] is not the correct explanation to [A].
4. [A] is false and [R] is true .

Q. Which of the following(s) process(es) can be used in separation of particles in downstream processing?

1. Filtration
2. Centrifugation
3. Floculation
4. Distillation

Q. Damage to structure and biological activity of product is not desirable during downstream processing.

1. True
2. False

Q. Cell disintegration during downstream processing is result of ________.

1. Stirring of cells, to prevent clumping, for better growth
2. Rupturing of the cell walls and/or cell membranes to release product of interest formed within the cell
3. Clumping of cells using chemicals for better separation
4. None of the above

Q. Assertion: Disintegration of cells is performed using gentle chemical, physical and enzymatic methods.
Reason: Product of interest must not be damaged during cell disintegration.

1. Both [A] and [R] are true and [R] is a correct explanation to [A].
2. [A] is true and [R] is false.
3. Both [A] and [R] are true but [R] is not the correct explanation to [A].
4. [A] is false and [R] is true .

Q. Disintegration of cell is required when the product of interest is .

1. intracellular
2. intercellular
3. extracellular

Q. Damage to structure and biological activity of product is not desirable during downstream processing.

1. False
2. True

Q. Choose the correct pairs from various methods to disintegrate cells with their respective examples:

1. Physical
2. Enzymatic
3. Lysozyme
4. Alkalis
5. Heat Shock
6. Chemical

Q. Assertion: Disintegration of cells is performed using gentle chemical, physical and enzymatic methods.
Reason: Product of interest must not be damaged during cell disintegration.

1. [A] is true and [R] is false.
2. [A] is false and [R] is true .
3. Both [A] and [R] are true and [R] is a correct explanation to [A].
4. Both [A] and [R] are true but [R] is not the correct explanation to [A].

Q. Disintegration of cell is required when the product of interest is .

1. intracellular
2. intercellular
3. extracellular

Q. The technique used to sterilize nutrient media is

1. Laminar airflow
2. Autoclave
3. Hot air oven
4. Centrifugation

Q. Isopycnic centrifugation is also known as

1. Density gradient centrifugation
2. Zonal centrifugation
3. Differential centrifugation
4. All of the above

Q. Cell disintegration during downstream processing is result of ________.

1. Stirring cells to prevent clumping for better growth
2. Clumping cells using chemicals for better separation
3. Rupturing cell walls and/or cell membranes to release product of interest formed within the cell
4. None of the above