Extraction of Genomic DNA

Q.

What is the recombinant DNA technology process?

Q. Which of the following genes were introduced in cotton to protect it from cotton bollworms?

1. cryAc and cryab
2. cryIAc and cryIAb
3. cryIAc and cryIIAb
4. nif genes

Q. Restriction fragment length polymorphism (RFLP) is

1. the technique used to fingerprint patterns of inheritance
2. the difference in the restriction maps between the two alleles in a diploid cell
3. the difference in the restriction maps between two individuals of one species
4. all the above

Q. The acid used for preliminary separation of biomolecules in a living tissue is

1. Trichlorobenzoic acid
2. Benzoic acid
3. Acetic acid
4. Trichloroacetic acid

Q. Identify the components labelled A, B, C, D and E in the diagram (cell membrane) below from the list (i) to (vii) given along with-
Components:-
(i) Sugar (ii) Protein (iii) Lipid bilayer
(iv) Integral protein (v) Cytoplasm (vi) Cell wall
(vii) External protein
The correct components are-

1. A-(i), B-(ii), C-(iii), D-(iv), E-(v)
2. A-(ii), B-(i), C-(iii), D-(iv), E-(v)
3. A-(i), B-(ii), C-(iii), D-(iv), E-(vi)
4. A-(i), B-(ii), C-(iii), D-(vii), E-(v)

Q.

Give three uses of plasmolysis in our daily life.

Q. Which of the following is not a step in PCR?
a) Denaturation
b) Amplification
c) Annealing
d) Extension
[1 mark]

Q. The molecules in the membrane that limit its permeability are the:

1. Carbohydrates
2. Phospholipids
3. Proteins
4. Water

Q.

Insect resistance transgenic cotton has been produced by inserting a piece of DNA from

1. A bacterium

2. A wild relative of cotton

3. A virus

4. An insect

Q.

How is recombinant DNA produced?

Q.

Is DNA prokaryotic or eukaryotic?

Q. Cell membrane is made up of a phospholipid bilayer, with the phosphate group forming the head and the lipid chain forming the tail.

1. True
2. False

Q. The lipid molecules present in plasma membrane have polar heads and non-polar tails (as shown in figure). Which option represents the correct arrangement of lipids in lipid bilayer?

1. 
2. 
3. 
4. 

Q. Chilled ethanol is used in biotechnology for:

1. Creating transient pores in cell membrane
2. Cell culture preparation
3. DNA precipitation
4. Protoplast fusion

Q. Select the option that correctly identifies the given statements as true (T) or false (F)

I. A bacteria that is helpful in creating transgenic plants is Agrobacterium

II. Silencing of mRNA or RNA interference (RNAi) technique is used to produce transgenic plants resistant to nematodes

III. The Ti plasmid is used for introducing foreign DNA in plants, and is found in Azotobacter

1. I - T, II - F, III - T
2. I - F, II - T, III - F
3. I - T, II - T, III - F
4. I - T, II - F, III - F

Q. Which of the following is incorrect regarding gel electrophoresis after PCR amplification of gene of interest?

1. Determination of location of DNA with gene of interest on the gel requires the presence of ladder
2. Determination of location of DNA with gene of interest on the gel does not require the presence of ladder
3. Markers are present in the form of ladder which signify molecular weight of DNA molecules
4. Markers contain DNA fragments of known molecular weight which get separated to form known standards

Q. Which of the following substances move faster through cell membrane?

1. lipid solubility
2. hydrophilic moiety
3. more solubility in water
4. the size of large proteins

Q. The length of an nascent mRNA is 1000 nucleotides. How many phosphate groups are present?

1. 1002
2. 1003
3. 1000
4. 2006

Q. Avery, MacLeod and McCarty observed that in the experimental tubes to which RNAse and proteinase were added, the transformation could happen. This indicated that:

1. RNA is the transforming principle
2. Protein is the transforming principle
3. Neither RNA nor protein could be the genetic material
4. Either RNA or protein could be the genetic material

Q.

Write the correct terms/options for the movement of particles.

Water from less concentrated solution to more concentrated solution.

Q.
(a) Name the gene used to develop Bt cotton.
(b) Where is the above gene obtained from?
(c) What converts the inactive Bt protoxin into its active form?
[3 marks]

Q. It is more difficult to digest plant cells than animal cells due to the presence of cell wall in plant cells.

1. False
2. True

Q. DNA isolated from cells cannot be seen through naked eyes.

1. True
2. False

Q. Purified DNA ultimately precipitate out after the addition of chilled ethanol. This DNA that separate out can be removed by _____________.

1. Electrophoresis
2. Downstream processing
3. PCR
4. Spooling

Q. First step in genetic engineering is

1. Isolation of RNA
2. Isolation of protein
3. Isolation of genetic material
4. Purification of protein

Q. During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

1. DNA
2. RNA
3. Polysaccharides
4. Histones

Q.

Introduction of dna into bacteria by exposing the cell to the milli seconds jolts of ten thousand volt is

Q. Mention the different steps of process of recombinant DNA technology.

Q. ‘Sandwich Model’ for cell membrane was given by __________.

1. Davson and Danielli(1935)
2. Singer and Nicolson (1972)
3. Gorter and Grendel (1925)
4. Overton (1875)

Q. Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombination DNA technology.