Extraction of Genomic DNA

Q. First step in genetic engineering is

1. Isolation of RNA
2. Isolation of protein
3. Isolation of genetic material
4. Purification of protein

Q. Rupturing of the cell and digestion of the histone does not provide DNA. Why?

1. DNA stays dissolved in water
2. Salt needs to be added for DNA to be extracted
3. DNA cannot be clumped in aqueous solution
4. DNA needs alcohol to be precipitated

Q. Detergent is used to break open the cell membrane. How does detergent aid in this process?

1. Cell membrane reacts with the acidic detergent and is dissolved in it
2. Detergent breaks the cell membrane after binding to the hydrophobic lipid tails
3. Detergent can make holes in the cell membrane for the DNA to come out
4. Detergent dissolves the cell membrane because of its alkalinity

Q. Histone is a protein that primarily works as a DNA packaging molecule.

1. True
2. False

Q. When using split pea for DNA extraction, chitinase can be used for lysing the cell wall of the pea cells.

1. True
2. False

Q. To make DNA molecules to stick and get clumped, __ is added.

Q. Cell membrane is made up of a phospholipid bilayer with the phosphate group forming the head and the lipid chain forming the tail.

1. True
2. False

Q. If proteases are not used during DNA extraction, what would be the end result?

1. DNA would be cut into small pieces by nucleases
2. Helicase can unwind the DNA once the cell is ruptured
3. DNA can bind to certain proteins once it is out of nuclear membrane and hence they need to be destroyed
4. DNA is bound with histone proteins that needs to be digested

Q. It is more difficult to digest plant cells than animal cells due to the presence of cell wall in plant cells.

1. False
2. True

Q. DNA isolated from cells cannot be seen through naked eyes.

1. True
2. False