Gene Regulation

Q. In eukaryotes, regulation of gene expression could be exerted at:

1. Transcriptional level
2. Translation level
3. Transport of mRNA from nucleus to cytoplasm
4. All the above

Q. Which of the following is not a feature of eukaryotic gene expression?

1. Many genes are interrupted by noncoding DNA sequences
2. mRNA is often extensively modified before translation
3. Polycistronic mRNA are very rare
4. RNA synthesis and protein synthesis are coupled

Q. Choose the odd one out with respect to the transcription unit.

1. Promoter
2. Operator
3. Structural gene
4. Terminator

Q.

What are the steps in gene expression?

Q.

What is the difference between positive and negative gene regulation?

Q. In operon model, regulator gene functions as

Q. Which of the following process(es) is not involved as a step in DNA fingerprinting?

1. Southern blotting
2. Gel electrophoresis
3. DNA transcription
4. Both a and b

Q. A gene of operon which forms the repressor protein is

1. Structural
2. Promoter
3. Operator
4. Regulator

Q. Which of the following statements are correct?
I. rRNA provides the template for synthesis of proteins.
II. tRNA brings amino acids and reads the genetic code.
III. RNA polymerase associates with the sigma factor to initiate transcription.
IV. A segment of DNA coding for polypeptide is called intron.

1. I and III
2. I and II
3. I, II and III
4. II and III

Q. Assertion: In eukaryotes, transcription occurs in nucleus
Reason : In bacteria, transcription and translation occurs in cytoplasm.

1. Both assertion and reason are true and reason is the correct explanation of assertion
2. Both assertion and reason are true but reason is not the correct explanation of assertion
3. Both assertion and reason are false
4. Assertion is true but reason is false

Q.

Which of the following is correct:

(a) Cells of all living organisms have a nucleus.

(b) Both animal and plant cells have a well defined cell wall.

(c) In prokaryotes, there are no membrane bound organelles.

(d) Cells are formed de novo from abiotic materials.

Q. In eukaryotes, regulation of gene expression could be exerted at:

1. Transcriptional level
2. Translational level
3. Transport of mRNA from nucleus to cytoplasm
4. All of the above

Q.

What is an outcome of gene regulation?

Q. The Rho protein helps to:

1. to stop translation

2. to terminate transcription
3. to bind RNA polymerase to the DNA
4. to help RNA polymerase finding the promoter site

Q.

At what levels can genes be regulated in eukaryotes? [2]

Q.

What controls gene expression in eukaryotes?

Q.

What factors are involved in the regulation of gene expression?

Q.

What is he difference between gene expression and gene regulation?

Q.

What does gene expression depend on?

Q. In transcription, against which strand of DNA does the DNA dependent RNA polymerase catalyse the process?

1. Template strand
2. Coding strand
3. Alpha strand
4. Sense strand

Q.

What is the primary step in gene regulation?

Q. Which of the following controls the rate of m – RNA synthesis ?

1. Operator gene
2. Structural gene
3. Promoter gene
4. Regulator gene

Q. During transcription, RNA polymerase first binds to which region of the transcription unit?

1. promoter
2. enhancer
3. regulator
4. motifs

Q. Gene expression results in formation of

1. protein
2. mRNA
3. Amino acids
4. Operons

Q.

What is gene expression in simple terms?

Q. In the lac operon of E.coli, which of the following happens when the repressor binds to the operator?

1. Transcription occurs
2. Transcription is blocked
3. RNA polymerase binds to the promoter
4. Transcription is slowed down

Q. All of the following are found in prokaryotic messenger RNA except

1. UGA codon
2. AUG
3. introns
4. uracil

Q.

What causes genes to be turned on or off?

Q. Inducible operons differ from repressible operons in that

1. operator of inducible operons is switched off by a repressor protein
2. inducible operons have an operator that controls binding of RNA polymerase to the promoter region
3. inducible operons use activators and positive control to regulate their transcription
4. inducible operons usually include structural genes that function in catabolic pathways

Q. Match the following regarding transcription:

p	Initiation	i	Ribonucleotides are polymerised according to the code
q	Elongation	ii	RNA polymerase binds to promoter
r	Termination	iii	mRNA is bound by protective molecules
s	Capping	iv	RNA polymerase detaches from DNA

1. p-i, q-ii, r-iii, s-iv
2. p-ii, q-iii, r-iv, s-i
3. p-ii, q-i, r-iv, s-iii
4. p-iv, q-iii, r-ii, s-i